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Sep 26, 2018· When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously expanding and undergoing adaptations.

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In general, vectors should have a set of characteristics that make them suitable for transformation and selection in the host organism. The first component is the origin of replication (ori) that will be recognized by the cellular replication machinery and will also define the number of copies of a given plasmid in the cell.Replication origins are usually recognized by their specific organism ...

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